The project started in Feb 2017.
Soluble biomarkers indicative of bacterial translocation in SpA
- lipopolyaccharide binding protein (LBP) is upregulated in axial SpA patients compa- red to controls.
- there is no difference according to disease state ( i.e. nr-axial SpA and AS) and disease activity (i.e. BASDAIhigh and BASDAI low).
- In patients from GIANT cohort (Belgium) LBP serum levels were significantly higher in patients with chronic gut inflammation compared to patients without gut inflammation.
In transcriptome analysis of CD14+ monocy- tes 957 Affymetrix probe sets were differen- tially expressed between axSpA patients and HC (Berlin). Coexpression analysis with refe- rence transcriptomes found an overlap of these IDs with late myeolopoesis and responses trigged by G-CFS mobilization and by LPS and TNF suggesting changes in myelopoiesis.
Mechanism of translocation in HLA-B27 tg rats
- HLAB27tg rats spontaneously develop coli- tis as indicted by infiltration of CD3+ T cells.
- mRNA expression data of colon epithelial cells suggest dysregulation of tight junction molecules in HLA-B27tg rats. These differences could not be verified on protein level suggesting that translocation may occur despite unimpaired expression of tight junction molecules